Zscan4 is expressed specifically during late meiotic prophase in both spermatogenesis and oogenesis (2022)

Abstract

Mouse zinc finger and SCAN domain containing 4 (Zscan4) proteins, which are encoded by multiple copies of Zscan4 genes, are expressed specifically in preimplantation embryos in vivo and embryonic stem (ES) cells in vitro. However, the expression patterns of mouse Zscan4 in vivo have been largely elusive. Here, we show that Zscan4 proteins are expressed in adult ovaries and testes. In ovaries, Zscan4 proteins were detected in germinal vesicle (GV) stage oocytes in antral follicles, indicating that Zscan4 genes are activated during the diplotene/dictyate stage in meiotic prophase I. Remarkably, Zscan4 showed different spatial localization patterns between two distinct GV oocytes, which can be distinguished by global chromatin organization—surrounded nucleolus (SN) and non-surrounded nucleolus (NSN). These spatiotemporal differences in Zscan4 localizations correlated with the transition of RNA polymerase II-mediated transcriptional status during GV oocyte maturation. In testes, Zscan4 proteins were detected in spermatocytes at late pachytene/diplotene stages and in Sertoli cells. These results suggest that Zscan4 may play critical roles during late meiotic prophase in both males and females.

Original languageEnglish
Pages (from-to)167-178
Number of pages12
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume53
Issue number2
DOIs
Publication statusPublished - 2017 Feb 1

Keywords

  • ES cell
  • GV oocyte
  • Ovary
  • Preimplantation embryo
  • SN and NSN oocyte
  • Testis

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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Ishiguro, K. I., Monti, M., Akiyama, T., Kimura, H., Chikazawa-Nohtomi, N., Sakota, M., Sato, S., Redi, C. A., Ko, S., & Ko, M. S. H. (2017). Zscan4 is expressed specifically during late meiotic prophase in both spermatogenesis and oogenesis. In Vitro Cellular and Developmental Biology - Animal, 53(2), 167-178. https://doi.org/10.1007/s11626-016-0096-z

Zscan4 is expressed specifically during late meiotic prophase in both spermatogenesis and oogenesis. / Ishiguro, Kei ichiro; Monti, Manuela; Akiyama, Tomohiko et al.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 53, No. 2, 01.02.2017, p. 167-178.

Research output: Contribution to journalArticlepeer-review

Ishiguro, KI, Monti, M, Akiyama, T, Kimura, H, Chikazawa-Nohtomi, N, Sakota, M, Sato, S, Redi, CA, Ko, S & Ko, MSH 2017, 'Zscan4 is expressed specifically during late meiotic prophase in both spermatogenesis and oogenesis', In Vitro Cellular and Developmental Biology - Animal, vol. 53, no. 2, pp. 167-178. https://doi.org/10.1007/s11626-016-0096-z

Ishiguro KI, Monti M, Akiyama T, Kimura H, Chikazawa-Nohtomi N, Sakota M et al. Zscan4 is expressed specifically during late meiotic prophase in both spermatogenesis and oogenesis. In Vitro Cellular and Developmental Biology - Animal. 2017 Feb 1;53(2):167-178. https://doi.org/10.1007/s11626-016-0096-z

Ishiguro, Kei ichiro ; Monti, Manuela ; Akiyama, Tomohiko et al. / Zscan4 is expressed specifically during late meiotic prophase in both spermatogenesis and oogenesis. In: In Vitro Cellular and Developmental Biology - Animal. 2017 ; Vol. 53, No. 2. pp. 167-178.

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title = "Zscan4 is expressed specifically during late meiotic prophase in both spermatogenesis and oogenesis",

abstract = "Mouse zinc finger and SCAN domain containing 4 (Zscan4) proteins, which are encoded by multiple copies of Zscan4 genes, are expressed specifically in preimplantation embryos in vivo and embryonic stem (ES) cells in vitro. However, the expression patterns of mouse Zscan4 in vivo have been largely elusive. Here, we show that Zscan4 proteins are expressed in adult ovaries and testes. In ovaries, Zscan4 proteins were detected in germinal vesicle (GV) stage oocytes in antral follicles, indicating that Zscan4 genes are activated during the diplotene/dictyate stage in meiotic prophase I. Remarkably, Zscan4 showed different spatial localization patterns between two distinct GV oocytes, which can be distinguished by global chromatin organization—surrounded nucleolus (SN) and non-surrounded nucleolus (NSN). These spatiotemporal differences in Zscan4 localizations correlated with the transition of RNA polymerase II-mediated transcriptional status during GV oocyte maturation. In testes, Zscan4 proteins were detected in spermatocytes at late pachytene/diplotene stages and in Sertoli cells. These results suggest that Zscan4 may play critical roles during late meiotic prophase in both males and females.",

keywords = "ES cell, GV oocyte, Ovary, Preimplantation embryo, SN and NSN oocyte, Testis",

author = "Ishiguro, {Kei ichiro} and Manuela Monti and Tomohiko Akiyama and Hiromi Kimura and Nana Chikazawa-Nohtomi and Miki Sakota and Saeko Sato and Redi, {Carlo Alberto} and Shigeru Ko and Ko, {Minoru S.H.}",

note = "Funding Information: We thank Drs. Alberto Calligaro and Antonia Icaro Cornaglia for their help in the adult ovarian section preparation. We also thank Collaborative Research Resources, School of Medicine, Keio University for technical assistance and all members of the Ko laboratory for their support and discussion. This work was supported in part by a Grant-in-Aid for Scientific Research on Innovative Areas “Mechanisms regulating gamete formation in animals” (KAKENHI no. 16H01257); a Grant-in-Aid for Scientific Research on Innovative Areas “Epigenome dynamics and regulation in germ cells” (KAKENHI no. 16H01221); a Grant-in-Aid Scientific Research (C) (KAKENHI no. 26440003); a Grant-in-Aid for challenging Exploratory Research (KAKENHI no. 15606842) from MEXT, Japan; the Takeda Science Foundation; the Uehara Memorial Foundation; the Mochida Memorial Foundation for Medical and Pharmaceutical Research; the Yamada Science Foundation (to K.I.); and a Grant-in-Aid for challenging Exploratory Research (KAKENHI no. 15606842) from MEXT, Japan (to M.S.H.K.). Publisher Copyright: {\textcopyright} 2016, The Author(s).",

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AU - Kimura, Hiromi

AU - Chikazawa-Nohtomi, Nana

AU - Sakota, Miki

AU - Sato, Saeko

AU - Redi, Carlo Alberto

AU - Ko, Shigeru

AU - Ko, Minoru S.H.

N1 - Funding Information:We thank Drs. Alberto Calligaro and Antonia Icaro Cornaglia for their help in the adult ovarian section preparation. We also thank Collaborative Research Resources, School of Medicine, Keio University for technical assistance and all members of the Ko laboratory for their support and discussion. This work was supported in part by a Grant-in-Aid for Scientific Research on Innovative Areas “Mechanisms regulating gamete formation in animals” (KAKENHI no. 16H01257); a Grant-in-Aid for Scientific Research on Innovative Areas “Epigenome dynamics and regulation in germ cells” (KAKENHI no. 16H01221); a Grant-in-Aid Scientific Research (C) (KAKENHI no. 26440003); a Grant-in-Aid for challenging Exploratory Research (KAKENHI no. 15606842) from MEXT, Japan; the Takeda Science Foundation; the Uehara Memorial Foundation; the Mochida Memorial Foundation for Medical and Pharmaceutical Research; the Yamada Science Foundation (to K.I.); and a Grant-in-Aid for challenging Exploratory Research (KAKENHI no. 15606842) from MEXT, Japan (to M.S.H.K.). Publisher Copyright:© 2016, The Author(s).

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AB - Mouse zinc finger and SCAN domain containing 4 (Zscan4) proteins, which are encoded by multiple copies of Zscan4 genes, are expressed specifically in preimplantation embryos in vivo and embryonic stem (ES) cells in vitro. However, the expression patterns of mouse Zscan4 in vivo have been largely elusive. Here, we show that Zscan4 proteins are expressed in adult ovaries and testes. In ovaries, Zscan4 proteins were detected in germinal vesicle (GV) stage oocytes in antral follicles, indicating that Zscan4 genes are activated during the diplotene/dictyate stage in meiotic prophase I. Remarkably, Zscan4 showed different spatial localization patterns between two distinct GV oocytes, which can be distinguished by global chromatin organization—surrounded nucleolus (SN) and non-surrounded nucleolus (NSN). These spatiotemporal differences in Zscan4 localizations correlated with the transition of RNA polymerase II-mediated transcriptional status during GV oocyte maturation. In testes, Zscan4 proteins were detected in spermatocytes at late pachytene/diplotene stages and in Sertoli cells. These results suggest that Zscan4 may play critical roles during late meiotic prophase in both males and females.

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